Cell Reports (Aug 2020)

Transite: A Computational Motif-Based Analysis Platform That Identifies RNA-Binding Proteins Modulating Changes in Gene Expression

  • Konstantin Krismer,
  • Molly A. Bird,
  • Shohreh Varmeh,
  • Erika D. Handly,
  • Anna Gattinger,
  • Thomas Bernwinkler,
  • Daniel A. Anderson,
  • Andreas Heinzel,
  • Brian A. Joughin,
  • Yi Wen Kong,
  • Ian G. Cannell,
  • Michael B. Yaffe

Journal volume & issue
Vol. 32, no. 8
p. 108064

Abstract

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Summary: RNA-binding proteins (RBPs) play critical roles in regulating gene expression by modulating splicing, RNA stability, and protein translation. Stimulus-induced alterations in RBP function contribute to global changes in gene expression, but identifying which RBPs are responsible for the observed changes remains an unmet need. Here, we present Transite, a computational approach that systematically infers RBPs influencing gene expression through changes in RNA stability and degradation. As a proof of principle, we apply Transite to RNA expression data from human patients with non-small-cell lung cancer whose tumors were sampled at diagnosis or after recurrence following treatment with platinum-based chemotherapy. Transite implicates known RBP regulators of the DNA damage response and identifies hnRNPC as a new modulator of chemotherapeutic resistance, which we subsequently validated experimentally. Transite serves as a framework for the identification of RBPs that drive cell-state transitions and adds additional value to the vast collection of publicly available gene expression datasets.

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