Evaluation of the Antioxidant, Antimicrobial, and Anti-Biofilm Effects of the Stem Bark, Leaf, and Seed Extracts from <i>Hymenaea courbaril</i> and Characterization by UPLC-ESI-QTOF-MS/MS Analysis
Jhonatas Emílio Ribeiro da Cruz,
Hellyssa Cataryna Saldanha,
Andressa Moreira do Nascimento,
Rafaela Barbosa Borges,
Marcos de Souza Gomes,
Guilherme Ramos Oliveira e Freitas,
Carla Monteiro Leal,
Everton Allan Ferreira,
Ademar Alves da Silva Filho,
Enyara Rezende Morais
Affiliations
Jhonatas Emílio Ribeiro da Cruz
Institute of Biotechnology, Federal University of Uberlândia, Campus Patos de Minas, Rua Major Jerônimo, 566, sala 205, Patos de Minas 38700-002, MG, Brazil
Hellyssa Cataryna Saldanha
Institute of Biotechnology, Federal University of Uberlândia, Campus Patos de Minas, Rua Major Jerônimo, 566, sala 205, Patos de Minas 38700-002, MG, Brazil
Andressa Moreira do Nascimento
Institute of Biotechnology, Federal University of Uberlândia, Campus Patos de Minas, Rua Major Jerônimo, 566, sala 205, Patos de Minas 38700-002, MG, Brazil
Rafaela Barbosa Borges
Institute of Biotechnology, Federal University of Uberlândia, Campus Patos de Minas, Rua Major Jerônimo, 566, sala 205, Patos de Minas 38700-002, MG, Brazil
Marcos de Souza Gomes
Institute of Chemistry, Federal University of Uberlândia, Campus Patos de Minas, Rua Major Jerônimo, 566, Patos de Minas 38700-002, MG, Brazil
Guilherme Ramos Oliveira e Freitas
Institute of Biotechnology, Federal University of Uberlândia, Campus Patos de Minas, Rua Major Jerônimo, 566, sala 205, Patos de Minas 38700-002, MG, Brazil
Carla Monteiro Leal
Faculty of Pharmacy, Department of Pharmaceutical Sciences, Federal University of Juiz de Fora, Rua José Lourenço Kelmer, s/n, Juiz de Fora 36036-900, MG, Brazil
Everton Allan Ferreira
Faculty of Pharmacy, Department of Pharmaceutical Sciences, Federal University of Juiz de Fora, Rua José Lourenço Kelmer, s/n, Juiz de Fora 36036-900, MG, Brazil
Ademar Alves da Silva Filho
Faculty of Pharmacy, Department of Pharmaceutical Sciences, Federal University of Juiz de Fora, Rua José Lourenço Kelmer, s/n, Juiz de Fora 36036-900, MG, Brazil
Enyara Rezende Morais
Institute of Biotechnology, Federal University of Uberlândia, Campus Patos de Minas, Rua Major Jerônimo, 566, sala 205, Patos de Minas 38700-002, MG, Brazil
Currently, biofilm-forming bacteria are difficult to treat by conventional antibiotic therapy and are, thus, becoming a clinical and epidemiological problem worldwide. Medicinal plants have been identified as novel alternative treatments due to their therapeutic and antimicrobial effects. In this context, the present study aimed to determine the total phenolic content, antioxidant capacity, and antimicrobial and anti-biofilm potential of nine extracts of Hymenaea courbaril (Fabaceae), popularly known as Jatobá. Furthermore, extracts that exhibited biofilm inhibitory activity against S. aureus (ATCC 25923) were selected for UPLC-HRMS/MS chemical analysis. Our results showed a high total phenolic content, mainly in the stem bark extract, and that the plant is rich in compounds with antioxidant activity. In the anti-biofilm analysis, leaf extracts stood out in comparison with chloramphenicol, with inhibition percentages of 78.29% and 78.85%, respectively. Through chemical analysis by UPLC-HRMS/MS, chrysoeriol-7-O-neohesperidoside, isorhamnetin-3-O-glucoside, and 3,7-di-O-methylquercetin were annotated for the first time in the leaves of H. courbaril. Therefore, these results showed the potential use of H. courbaril as an antioxidant and point to its use in antimicrobial therapy with an anti-biofilm effect.
