A Novel Chemoattractant Lectin, Karatoxin, From the Dorsal Spines of the Small Scorpionfish Hypodytes rubripinnis

Mitsuko Shinohara; Kuniko Nagasaka; Hideyuki Nakagawa; Kozue Edo; Hitomi Sakai; Koji Kato; Futoshi Iwaki; Kiyoshi Ohura; Haruhiko Sakuraba

Journal of Pharmacological Sciences (Jan 2010)

A Novel Chemoattractant Lectin, Karatoxin, From the Dorsal Spines of the Small Scorpionfish Hypodytes rubripinnis

Mitsuko Shinohara,
Kuniko Nagasaka,
Hideyuki Nakagawa,
Kozue Edo,
Hitomi Sakai,
Koji Kato,
Futoshi Iwaki,
Kiyoshi Ohura,
Haruhiko Sakuraba

Affiliations

Mitsuko Shinohara: Depatment of Pharmacology, Osaka Dental University, Hirakata 573-1121, Japan; Corresponding author. [email protected]
Kuniko Nagasaka: Fukae Nagasaka Clinic, Etajima 737-2214, Japan
Hideyuki Nakagawa: Depatment of Pharmacology, Osaka Dental University, Hirakata 573-1121, Japan; Department of Environmental Symbiosis, Institute of Socio-Arts and Sciences, The University of Tokushima Graduate School, Tokushima 770-8502, Japan
Kozue Edo: Department of Environmental Symbiosis, Institute of Socio-Arts and Sciences, The University of Tokushima Graduate School, Tokushima 770-8502, Japan
Hitomi Sakai: Department of Environmental Symbiosis, Institute of Socio-Arts and Sciences, The University of Tokushima Graduate School, Tokushima 770-8502, Japan
Koji Kato: Depatment of Pharmacology, Osaka Dental University, Hirakata 573-1121, Japan
Futoshi Iwaki: Depatment of Pharmacology, Osaka Dental University, Hirakata 573-1121, Japan
Kiyoshi Ohura: Depatment of Pharmacology, Osaka Dental University, Hirakata 573-1121, Japan
Haruhiko Sakuraba: Department of Applied Biological Sciences, Kagawa University, Kagawa 761-0795, Japan

Journal volume & issue: Vol. 113, no. 4
pp. 414 – 417

Abstract

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A chemoattractant lectin from the dorsal spines of the redfin velvetfish, Hypodytes rubripinnis, was isolated using a combination of affinity chromatography techniques. The glycoprotein, with a molecular mass of 110 kDa, is named Karatoxin. Karatoxin caused agglutination of rabbit erythrocytes. This agglutination was effectively inhibited by d-mannose. In addition, Karatoxin exhibited not only mitogenic activity in the presence of murine splenocytes, but also chemotactic activity in the presence of guinea-pig neutrophils and macrophages. Thus, Karatoxin appears to be a novel chemoattractant lectin. These results suggest that the redfin velvetfish Hypodytes rubripinnis may be a novel source of biologically active substances. Keywords:: chemoattractant lectin, Karatoxin, Hypodytes rubrinnis

Published in Journal of Pharmacological Sciences

ISSN: 1347-8613 (Print)
Publisher: Elsevier
Country of publisher: Japan
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.journals.elsevier.com/journal-of-pharmacological-sciences

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