Frontiers in Plant Science (Jul 2022)
GmWRKY21, a Soybean WRKY Transcription Factor Gene, Enhances the Tolerance to Aluminum Stress in Arabidopsis thaliana
- Zhenzhen Han,
- Zhenzhen Han,
- Zhenzhen Han,
- Zhenzhen Han,
- Zhenzhen Han,
- Jinyu Wang,
- Jinyu Wang,
- Jinyu Wang,
- Jinyu Wang,
- Jinyu Wang,
- Xinxin Wang,
- Xinxin Wang,
- Xinxin Wang,
- Xinxin Wang,
- Xinxin Wang,
- Xijia Zhang,
- Xijia Zhang,
- Xijia Zhang,
- Xijia Zhang,
- Xijia Zhang,
- Yanbo Cheng,
- Yanbo Cheng,
- Yanbo Cheng,
- Yanbo Cheng,
- Yanbo Cheng,
- Zhandong Cai,
- Zhandong Cai,
- Zhandong Cai,
- Zhandong Cai,
- Zhandong Cai,
- Hai Nian,
- Hai Nian,
- Hai Nian,
- Hai Nian,
- Hai Nian,
- Qibin Ma,
- Qibin Ma,
- Qibin Ma,
- Qibin Ma,
- Qibin Ma
Affiliations
- Zhenzhen Han
- The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Zhenzhen Han
- The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China
- Zhenzhen Han
- The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China
- Zhenzhen Han
- The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China
- Zhenzhen Han
- Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou, China
- Jinyu Wang
- The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Jinyu Wang
- The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China
- Jinyu Wang
- The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China
- Jinyu Wang
- The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China
- Jinyu Wang
- Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou, China
- Xinxin Wang
- The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Xinxin Wang
- The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China
- Xinxin Wang
- The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China
- Xinxin Wang
- The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China
- Xinxin Wang
- Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou, China
- Xijia Zhang
- The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Xijia Zhang
- The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China
- Xijia Zhang
- The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China
- Xijia Zhang
- The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China
- Xijia Zhang
- Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou, China
- Yanbo Cheng
- The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Yanbo Cheng
- The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China
- Yanbo Cheng
- The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China
- Yanbo Cheng
- The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China
- Yanbo Cheng
- Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou, China
- Zhandong Cai
- The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Zhandong Cai
- The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China
- Zhandong Cai
- The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China
- Zhandong Cai
- The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China
- Zhandong Cai
- Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou, China
- Hai Nian
- The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Hai Nian
- The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China
- Hai Nian
- The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China
- Hai Nian
- The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China
- Hai Nian
- Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou, China
- Qibin Ma
- The State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, China
- Qibin Ma
- The Key Laboratory of Plant Molecular Breeding of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou, China
- Qibin Ma
- The Guangdong Subcenter of the National Center for Soybean Improvement, College of Agriculture, South China Agricultural University, Guangzhou, China
- Qibin Ma
- The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology, South China Agricultural University, Guangzhou, China
- Qibin Ma
- Zengcheng Teaching and Research Bases, South China Agricultural University, Guangzhou, China
- DOI
- https://doi.org/10.3389/fpls.2022.833326
- Journal volume & issue
-
Vol. 13
Abstract
The WRKY transcription factors (TFs) are one of the largest families of TFs in plants and play multiple roles in plant growth and development and stress response. In this study, GmWRKY21 encoding a WRKY transcription factor was functionally characterized in Arabidopsis and soybean. The GmWRKY21 protein containing a highly conserved WRKY domain and a C2H2 zinc-finger structure is located in the nucleus and has the characteristics of transcriptional activation ability. The GmWRKY21 gene presented a constitutive expression pattern rich in the roots, leaves, and flowers of soybean with over 6-fold of relative expression levels and could be substantially induced by aluminum stress. As compared to the control, overexpression of GmWRKY21 in Arabidopsis increased the root growth of seedlings in transgenic lines under the AlCl3 concentrations of 25, 50, and 100 μM with higher proline and lower MDA accumulation. The results of quantitative real-time polymerase chain reaction (qRT-PCR) showed that the marker genes relative to aluminum stress including ALMT, ALS3, MATE, and STOP1 were induced in GmWRKY21 transgenic plants under AlCl3 treatment. The stress-related genes, such as KIN1, COR15A, COR15B, COR47, GLOS3, and RD29A, were also upregulated in GmWRKY21 transgenic Arabidopsis under aluminum stress. Similarly, stress-related genes, such as GmCOR47, GmDREB2A, GmMYB84, GmKIN1, GmGST1, and GmLEA, were upregulated in hair roots of GmWRKY21 transgenic plants. In summary, these results suggested that the GmWRKY21 transcription factor may promote the tolerance to aluminum stress mediated by the pathways regulating the expression of the acidic aluminum stress-responsive genes and abiotic stress-responsive genes.
Keywords
