The modified method for blood serum labile iron quantification

Abstract

The goal of the research was to develop and test the modification of biochemical method for blood plasma labile iron quantification. As the basis a standard method for plasma iron determination with color reagent ferrozine was taken. Modification essence consists in applying a neutral buffer which prevents the release of iron from the iron-transport protein. The method was tested on in vitro and in vivo models. In experiments with FeSO4 and FeCl3 solutions iron-ferrozine complex formation with maximum OD =562nm was registered. Iron solutions were prepared on 4% albumin at neutral pH. In rhabdomyolisis model experiment, while heme oxygenase activity increase, the pool of labile ferric iron in blood plasma of experimental animals was measured. Such iron was absent in the control group. The results indicate the modified method effectiveness and the possibility of its usage in experimental and clinical trials.

Keywords

• rhabdomyolysis, ferrozine, iron, transferrin