Protective effect of vitamin C against thiamethoxam-induced toxicity in male rats

Abstract

Background: Thiamethoxam (THM) is a neonicotinoid insecticide used to control different insect pests on fruits, vegetables, and field crops. The misuse and continuous exposure to THM cause many harmful effects on health and the reproductive system. Aim: This work aims to investigate the efficiency of vitamin C (vit C) in reducing or eliminating the harmful effects of THM on testes, liver, and kidney of male rats. Methods: Forty eight sexually mature male Wister albino rats (weight: 170-190 g; age: 10-11 weeks) randomly allocated into six groups (8 males/group). Control group was orally given distilled water, vit. C group orally treated with 200 mg/kg b.wt of vit C, group 1/10 of THM LD50 orally treated with 156.3 mg/kg b.wt of THM, group 1/20 of THM LD50 orally with 78.15 mg/kg b.wt of THM, group 1/10 of THM LD50 + vit. C orally treated with 156.3 mg/kg b.wt of THM + 200 mg/kg b.wt of vit. C, group 1/20 of THM LD50 + vit. C orally treated with 78.15 mg/kg b.wt of THM + 200 mg/kg b.wt of vit. C. All groups were treated for five days per week for a whole period of 58 days. Blood samples were collected at the end of the experiment and serum was extracted for liver and kidney function and antioxidants measurements. Reproductive organs (testis, epididymis, and seminal vesicles) were collected and weighted in the end of the experiment. Results: The results showed that groups exposed to 1/10 and 1/20 of THM LD50 significantly (p [Open Vet J 2023; 13(10.000): 1334-1345]

Keywords

• thiamethoxam
• vitamin c
• oxidative stress
• antioxidant