Autofluorescence and Metabotyping of Soybean Varieties Using Confocal Laser Microscopy and High-Resolution Mass Spectrometric Approaches
Mayya P. Razgonova,
Muhammad A. Navaz,
Ekaterina S. Butovets,
Ludmila M. Lukyanchuk,
Olga A. Chunikhina,
Sezai Ercişli,
Alexei N. Emelyanov,
Kirill S. Golokhvast
Affiliations
Mayya P. Razgonova
N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, 190000 Saint-Petersburg, Russia
Muhammad A. Navaz
Laboratory for Research and Application of Supercritical Fluid Technologies in Agro-Food Biotechnology, National Research Tomsk State University, Lenin Ave, 36, 634050 Tomsk, Russia
Ekaterina S. Butovets
Federal Scientific Center of Agricultural Biotechnologies of the Far East Named After A.K. Chaika, St. Volozhenina, 30, Timiryazevsky Village, 692539 Ussuriysk, Russia
Ludmila M. Lukyanchuk
Federal Scientific Center of Agricultural Biotechnologies of the Far East Named After A.K. Chaika, St. Volozhenina, 30, Timiryazevsky Village, 692539 Ussuriysk, Russia
Olga A. Chunikhina
N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, 190000 Saint-Petersburg, Russia
Sezai Ercişli
Department of Horticulture, Faculty of Agriculture, Ataturk University, Erzurum 25240, Türkiye
Alexei N. Emelyanov
Federal Scientific Center of Agricultural Biotechnologies of the Far East Named After A.K. Chaika, St. Volozhenina, 30, Timiryazevsky Village, 692539 Ussuriysk, Russia
Kirill S. Golokhvast
N.I. Vavilov All-Russian Institute of Plant Genetic Resources, B. Morskaya 42-44, 190000 Saint-Petersburg, Russia
This research examines a detailed metabolomic and comparative analysis of bioactive substances of soybean varieties: “Primorskaya-4”, “Primorskaya-86”, “Primorskaya-96”, “Locus”, “Sphere”, “Breeze”, “Namul”, and “Musson” by the laser confocal microscope CLSM 800 and the mass spectrometry of bioactive compounds by tandem mass spectrometry. The laser microscopy allowed us to clarify in detail the spatial arrangement of phenolic acids, flavonols, and anthocyanin contents in soybeans. Research has convincingly shown that the polyphenolic content of soybeans, and, in particular, the anthocyanins, are spatially localized mainly in the seed coat of soybeans. Tandem mass spectrometry was used to identify chemical constituents in soybean extracts. The results of initial studies revealed the presence of one hundred and fourteen compounds; sixty-nine of the target analytes were tentatively identified as compounds from polyphenol groups.
