Rhizosphere Bacterium <i>Rhodococcus</i> sp. P1Y Metabolizes Abscisic Acid to Form Dehydrovomifoliol
Oleg S. Yuzikhin,
Natalia E. Gogoleva,
Alexander I. Shaposhnikov,
Tatyana A. Konnova,
Elena V. Osipova,
Darya S. Syrova,
Elena A. Ermakova,
Valerii P. Shevchenko,
Igor Yu. Nagaev,
Konstantin V. Shevchenko,
Nikolay F. Myasoedov,
Vera I. Safronova,
Alexey L. Shavarda,
Anton A. Nizhnikov,
Andrey A. Belimov,
Yuri V. Gogolev
Affiliations
Oleg S. Yuzikhin
All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 Saint-Petersburg, Russia
Natalia E. Gogoleva
Kazan Institute of Biochemistry and Biophysics, Federal Research Center “Kazan Scientific Center of the RAS”, Lobachevsky Street, 2/31, 420111 Kazan, Tatarstan Republic, Russia
Alexander I. Shaposhnikov
All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 Saint-Petersburg, Russia
Tatyana A. Konnova
Laboratory of Plant Infectious Diseases, FRC Kazan Scientific Center of RAS, 420111 Kazan, Tatarstan Republic, Russia
Elena V. Osipova
Kazan Institute of Biochemistry and Biophysics, Federal Research Center “Kazan Scientific Center of the RAS”, Lobachevsky Street, 2/31, 420111 Kazan, Tatarstan Republic, Russia
Darya S. Syrova
All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 Saint-Petersburg, Russia
Elena A. Ermakova
Kazan Institute of Biochemistry and Biophysics, Federal Research Center “Kazan Scientific Center of the RAS”, Lobachevsky Street, 2/31, 420111 Kazan, Tatarstan Republic, Russia
Valerii P. Shevchenko
Institute of Molecular Genetics, Russian Academy of Sciences, Akademika Kurchatova Square, 2, 123182 Moscow, Russia
Igor Yu. Nagaev
Institute of Molecular Genetics, Russian Academy of Sciences, Akademika Kurchatova Square, 2, 123182 Moscow, Russia
Konstantin V. Shevchenko
Institute of Molecular Genetics, Russian Academy of Sciences, Akademika Kurchatova Square, 2, 123182 Moscow, Russia
Nikolay F. Myasoedov
Institute of Molecular Genetics, Russian Academy of Sciences, Akademika Kurchatova Square, 2, 123182 Moscow, Russia
Vera I. Safronova
All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 Saint-Petersburg, Russia
Alexey L. Shavarda
Department of Genetics and Biotechnology, Saint-Petersburg State University, University Embankment, 199034 Saint-Petersburg, Russia
Anton A. Nizhnikov
All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 Saint-Petersburg, Russia
Andrey A. Belimov
All-Russia Research Institute for Agricultural Microbiology, Podbelskogo sh. 3, Pushkin, 196608 Saint-Petersburg, Russia
Yuri V. Gogolev
Kazan Institute of Biochemistry and Biophysics, Federal Research Center “Kazan Scientific Center of the RAS”, Lobachevsky Street, 2/31, 420111 Kazan, Tatarstan Republic, Russia
The phytohormone abscisic acid (ABA) plays an important role in plant growth and in response to abiotic stress factors. At the same time, its accumulation in soil can negatively affect seed germination, inhibit root growth and increase plant sensitivity to pathogens. ABA is an inert compound resistant to spontaneous hydrolysis and its biological transformation is scarcely understood. Recently, the strain Rhodococcus sp. P1Y was described as a rhizosphere bacterium assimilating ABA as a sole carbon source in batch culture and affecting ABA concentrations in plant roots. In this work, the intermediate product of ABA decomposition by this bacterium was isolated and purified by preparative HPLC techniques. Proof that this compound belongs to ABA derivatives was carried out by measuring the molar radioactivity of the conversion products of this phytohormone labeled with tritium. The chemical structure of this compound was determined by instrumental techniques including high-resolution mass spectrometry, NMR spectrometry, FTIR and UV spectroscopies. As a result, the metabolite was identified as (4RS)-4-hydroxy-3,5,5-trimethyl-4-[(E)-3-oxobut-1-enyl]cyclohex-2-en-1-one (dehydrovomifoliol). Based on the data obtained, it was concluded that the pathway of bacterial degradation and assimilation of ABA begins with a gradual shortening of the acyl part of the molecule.
