A Study and Application of Biocatalytic Synthesis of (S)-N-Boc-3-hydroxypiperidine

Abstract

This paper first uses the environmental friendly whole cell of biocatalyst pichia pastoris SIT2014 to asymmetrically synthesized anti-tumor drug of chiral intermediate (S)-N-Boc-3-hydroxypiperidine.. Improve the final biocatalytic reduction yield to 85.4% based on the study of fermentation optimization and biocatalytic asymmetrical reduction system for pichia pastoris. The ee value of obtained reduction product (S)-N-Boc-3-hydroxypiperidine hits over 99%. The study of this article is a successful case where the biocatalyst is applied to the green synthesis of chiral intermediate of anti-tumor drug.

Keywords

• pichia pastoris
• biocatalyst
• (S)-N-Boc-3-hydroxypiperidine
• asymmetrical reduction