Synthetic chimeric nucleases function for efficient genome editing

R. M. Liu; L. L. Liang; E. Freed; H. Chang; E. Oh; Z. Y. Liu; A. Garst; C. A. Eckert; R. T. Gill

doi:10.1038/s41467-019-13500-y

Nature Communications (Dec 2019)

Synthetic chimeric nucleases function for efficient genome editing

R. M. Liu,
L. L. Liang,
E. Freed,
H. Chang,
E. Oh,
Z. Y. Liu,
A. Garst,
C. A. Eckert,
R. T. Gill

Affiliations

R. M. Liu: Renewable and Sustainable Energy Institute (RASEI), University of Colorado
L. L. Liang: Renewable and Sustainable Energy Institute (RASEI), University of Colorado
E. Freed: Renewable and Sustainable Energy Institute (RASEI), University of Colorado
H. Chang: Department of Biochemistry, University of Colorado
E. Oh: Renewable and Sustainable Energy Institute (RASEI), University of Colorado
Z. Y. Liu: Department of Biochemistry, University of Colorado
A. Garst: Inscripta, Inc.
C. A. Eckert: Renewable and Sustainable Energy Institute (RASEI), University of Colorado
R. T. Gill: Renewable and Sustainable Energy Institute (RASEI), University of Colorado

DOI: https://doi.org/10.1038/s41467-019-13500-y
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 11

Abstract

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CRISPR-Cas systems have well characterized, modular structures. Here the authors use that architecture to design a Cas12a library of 560 synthetic chimeras, with altered PAM preferences and specificities.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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