Establishment of an induced pluripotent stem cell (SIAISi016-A) line from a 62-years old Chinese Han patient with Alzheimer's disease

Juan Huang; Ying Wang; Jian Zhao; Yulei Deng; Wenshi Wei

Stem Cell Research (Jun 2023)

Establishment of an induced pluripotent stem cell (SIAISi016-A) line from a 62-years old Chinese Han patient with Alzheimer's disease

Juan Huang,
Ying Wang,
Jian Zhao,
Yulei Deng,
Wenshi Wei

Affiliations

Juan Huang: Neurology Department, Huadong Hospital Affiliated to Fudan University, Shanghai, China
Ying Wang: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China; Shanghai Clinical Research and Trial Center, 201210 Shanghai, China
Jian Zhao: Lab of Regenerative Medicine and Neural Repair, Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai 201210, China; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China; Shanghai Clinical Research and Trial Center, 201210 Shanghai, China
Yulei Deng: Department of Neurology & Institute of Neurology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authors.
Wenshi Wei: Neurology Department, Huadong Hospital Affiliated to Fudan University, Shanghai, China; Corresponding authors.

Journal volume & issue: Vol. 69
p. 103091

Abstract

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A 62-years old Alzheimer's disease (AD) male patient donated his Peripheral blood mononuclear cells. The non-integrating episomal vector system used to reprogram PBMCs with Oct3/4, Klf4, Sox2 and c-Myc transcription factors. The pluripotency of transgene-free pluripotent stem cell (iPSC) was confirmed by immunocytochemistry for pluripotency markers-SOX2, NANOG, OCT3/4, SSEA4, TRA1-60, and TRA1-81. The differentiation capacity of the iPSCs into endoderm, mesoderm and ectoderm was assessed by AFP, SMA and βIII-TUBULIN, respectively. In addition, the iPSC line displayed a normal karyotype. This iPSC line might offer a good cell model to explore the pathological mechanisms and treatment strategies for AD.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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