Nature Communications (Sep 2016)
Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase
Abstract
Succinimide is a post-translational modification susceptible to rapid hydrolysis and generally associated with protein destabilisation. Here, the authors use mass spectroscopy to identify a stable succinimide intermediate that is responsible for the high thermostability of a thermophilic enzyme.