Nature Communications (Sep 2016)

Unexpected functional implication of a stable succinimide in the structural stability of Methanocaldococcus jannaschii glutaminase

  • Sanjeev Kumar,
  • Sunita Prakash,
  • Kallol Gupta,
  • Aparna Dongre,
  • Padmanabhan Balaram,
  • Hemalatha Balaram

DOI
https://doi.org/10.1038/ncomms12798
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 14

Abstract

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Succinimide is a post-translational modification susceptible to rapid hydrolysis and generally associated with protein destabilisation. Here, the authors use mass spectroscopy to identify a stable succinimide intermediate that is responsible for the high thermostability of a thermophilic enzyme.