Data on endogenous chicken sperm peptides and small proteins obtained through Top-Down High Resolution Mass Spectrometry
L. Soler,
V. Labas,
A. Thélie,
A.P. Teixeira-Gomes,
I. Grasseau,
L. Bouguereau,
E. Blesbois
Affiliations
L. Soler
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France
V. Labas
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France; INRA, Plateforme d’Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, 37380 Nouzilly, France; Corresponding author at: PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France.
A. Thélie
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France
A.P. Teixeira-Gomes
ISP, INRA, Université de Tours, 37380 Nouzilly, France; INRA, Plateforme d’Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, 37380 Nouzilly, France
I. Grasseau
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France
L. Bouguereau
ISP, INRA, Université de Tours, 37380 Nouzilly, France; INRA, Plateforme d’Analyse Intégrative des Biomolécules, Laboratoire de Spectrométrie de Masse, 37380 Nouzilly, France
E. Blesbois
PRC, INRA, CNRS, Université de Tours, IFCE, 37380 Nouzilly, France
The endogenous peptides and small proteins present in chicken sperm were identified in the context of the characterization of a fertility-diagnostic method based on the use of ICM-MS (Intact Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry). The interpretation and description of these data can be found in a research article, “Intact cell MALDI-TOF MS on sperm: a molecular test for male fertility diagnosis” (Soler et al., 2016) [1], and raw data derived from this analysis have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002768. Here, we describe the inventory of all the molecular species identified, along with their biochemical features and functional analysis. This peptide/protein catalogue can be further employed as reference for other studies and reveal that the use of proteomics allows for a global evaluation of sperm cells functions. Keywords: Chicken, Sperm, Top-Down HRMS, Peptidome
