Pressurized Liquid (PLE) Truffle Extracts Have Inhibitory Activity on Key Enzymes Related to Type 2 Diabetes (α-Glucosidase and α-Amylase)
Eva Tejedor-Calvo,
Diego Morales,
Laura Morillo,
Laura Vega,
Mercedes Caro,
Fhernanda Ribeiro Smiderle,
Marcello Iacomini,
Pedro Marco,
Cristina Soler-Rivas
Affiliations
Eva Tejedor-Calvo
Department of Production and Characterization of Novel Foods, Institute of Food Science Research—CIAL (UAM + CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain
Diego Morales
Department of Production and Characterization of Novel Foods, Institute of Food Science Research—CIAL (UAM + CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain
Laura Morillo
Department of Production and Characterization of Novel Foods, Institute of Food Science Research—CIAL (UAM + CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain
Laura Vega
Department of Production and Characterization of Novel Foods, Institute of Food Science Research—CIAL (UAM + CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain
Mercedes Caro
AZTI, Food Research, Basque Research and Technology Alliance (BRTA), Parque Tecnológico de Bizkaia, Astondo Bidea, Edificio 609, 48160 Derio, Spain
Fhernanda Ribeiro Smiderle
Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil
Marcello Iacomini
Department of Biochemistry and Molecular Biology, Federal University of Parana, Curitiba 81531-980, PR, Brazil
Pedro Marco
Department of Forest Resources, Agrifood Research and Technology Centre of Aragon (CITA), Agrifood Institute of Aragón—IA2 (CITA-Zaragoza University), Av. Montañana 930, 50059 Zaragoza, Spain
Cristina Soler-Rivas
Department of Production and Characterization of Novel Foods, Institute of Food Science Research—CIAL (UAM + CSIC), C/Nicolas Cabrera 9, Campus de Cantoblanco, Universidad Autónoma de Madrid, 28049 Madrid, Spain
An optimized PLE method was applied to several truffle species using three different solvent mixtures to obtain bioactive enriched fractions. The pressurized water extracts contained mainly (1 → 3),(1 → 6)-β-D-glucans, chitins, and heteropolymers with galactose and mannose in their structures. The ethanol extracts included fatty acids and fungal sterols and others such as brassicasterol and stigmasterol, depending on the species. They also showed a different fatty acid lipid profile depending on the solvent utilized and species considered. Ethanol:water extracts showed interesting lipids and many phenolic compounds; however, no synergic extraction of compounds was noticed. Some of the truffle extracts were able to inhibit enzymes related to type 2 diabetes; pressurized water extracts mainly inhibited the α-amylase enzyme, while ethanolic extracts were more able to inhibit α-glucosidase. Tuber brumale var. moschatum and T. aestivum var. uncinatum extracts showed an IC50 of 29.22 mg/mL towards α-amylase and 7.93 mg/mL towards α-glucosidase. Thus, use of the PLE method allows o bioactive enriched fractions to be obtained from truffles with antidiabetic properties.
