Cytotoxic and molecular assessment against breast (MCF-7) cancer cells with cobalt oxide nanoballs

Abstract

Among various types of cancer, the breast cancer is a very common and widely affected to the human beings especially female candidate. To keep this view the present work was designed to access the cytotoxic and apoptotic responses of breast (MCF-7) cancer cells with cobalt oxide nanoparticles (Co3O4NPs). The Co3O4NPs was prepared via solution process and well characterized. The materials crystallinity, size and phase were determined via XRD, whereas the morphology of Co3O4NPs was analyzed via SEM and TEM respectively. The Co3O4NPs were spherical in shape with an average diameter of (~33±1). The cytotoxicity rate of breast (MCF-7) cells was much influenced and dose dependent (1, 2, 5, 10, 25, 50 and 100 μL/mL) with Co3O4NPs confirmed via the MTT and NRU assays. The rate of ROS was increased gradually from 105, 124,132 and 148 with different concentrations of Co3O4NPs (10, 25, 50 and 100 μL/mL) with control. The mRNA level of apoptotic markers with Co3O4NPs was up-regulated and it shows the apoptosis in cells. The study describes the cytotoxicity with Co3O4NPs in breast (MCF-7) cancer cell were apoptotic gene expression with possible discussion.

Keywords

• Cobalt oxide NPs
• Cytotoxicity
• MCF-7 cells
• Reactive oxygen species
• RT-PCR