Nature Communications (Oct 2017)
Diversity oriented biosynthesis via accelerated evolution of modular gene clusters
- Aleksandra Wlodek,
- Steve G. Kendrew,
- Nigel J. Coates,
- Adam Hold,
- Joanna Pogwizd,
- Steven Rudder,
- Lesley S. Sheehan,
- Sarah J. Higginbotham,
- Anna E. Stanley-Smith,
- Tony Warneck,
- Mohammad Nur-E-Alam,
- Markus Radzom,
- Christine J. Martin,
- Lois Overvoorde,
- Markiyan Samborskyy,
- Silke Alt,
- Daniel Heine,
- Guy T. Carter,
- Edmund I. Graziani,
- Frank E. Koehn,
- Leonard McDonald,
- Alexander Alanine,
- Rosa María Rodríguez Sarmiento,
- Suzan Keen Chao,
- Hasane Ratni,
- Lucinda Steward,
- Isobel H. Norville,
- Mitali Sarkar-Tyson,
- Steven J. Moss,
- Peter F. Leadlay,
- Barrie Wilkinson,
- Matthew A. Gregory
Affiliations
- Aleksandra Wlodek
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Steve G. Kendrew
- Biotica Technology Ltd., Chesterford Research Park
- Nigel J. Coates
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Adam Hold
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Joanna Pogwizd
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Steven Rudder
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Lesley S. Sheehan
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Sarah J. Higginbotham
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Anna E. Stanley-Smith
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Tony Warneck
- Biotica Technology Ltd., Chesterford Research Park
- Mohammad Nur-E-Alam
- Biotica Technology Ltd., Chesterford Research Park
- Markus Radzom
- Biotica Technology Ltd., Chesterford Research Park
- Christine J. Martin
- Biotica Technology Ltd., Chesterford Research Park
- Lois Overvoorde
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Markiyan Samborskyy
- Department of Biochemistry, University of Cambridge
- Silke Alt
- Department of Molecular Microbiology, John Innes Centre, Norwich Research Park
- Daniel Heine
- Department of Molecular Microbiology, John Innes Centre, Norwich Research Park
- Guy T. Carter
- Chemical and Screening Sciences, Wyeth Pharmaceuticals
- Edmund I. Graziani
- Chemical and Screening Sciences, Wyeth Pharmaceuticals
- Frank E. Koehn
- Chemical and Screening Sciences, Wyeth Pharmaceuticals
- Leonard McDonald
- Chemical and Screening Sciences, Wyeth Pharmaceuticals
- Alexander Alanine
- Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED)
- Rosa María Rodríguez Sarmiento
- Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED)
- Suzan Keen Chao
- Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED)
- Hasane Ratni
- Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED)
- Lucinda Steward
- Roche Innovation Center Basel, Pharmaceutical Research and Early Development (PRED)
- Isobel H. Norville
- Defence Science and Technology Laboratory
- Mitali Sarkar-Tyson
- Defence Science and Technology Laboratory
- Steven J. Moss
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Peter F. Leadlay
- Department of Biochemistry, University of Cambridge
- Barrie Wilkinson
- Isomerase Therapeutics Ltd., Chesterford Research Park
- Matthew A. Gregory
- Isomerase Therapeutics Ltd., Chesterford Research Park
- DOI
- https://doi.org/10.1038/s41467-017-01344-3
- Journal volume & issue
-
Vol. 8,
no. 1
pp. 1 – 10
Abstract
Reengineering polyketide synthase encoding genes to produce analogues of natural products can be slow and low-yielding. Here the authors use accelerated evolution to recombine the gene cluster for rapid production of rapamycin-related products.
