Investigation on Natural Infection of Covert Mortality Nodavirus in Farmed Giant Freshwater Prawn (<i>Macrobrachium rosenbergii</i>)
Jitao Xia,
Chong Wang,
Liang Yao,
Wei Wang,
Wenxiu Zhao,
Tianchang Jia,
Xingtong Yu,
Guoliang Yang,
Qingli Zhang
Affiliations
Jitao Xia
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Chong Wang
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Qingdao 266071, China
Liang Yao
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Wei Wang
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Wenxiu Zhao
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Tianchang Jia
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Xingtong Yu
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Key Laboratory of Maricultural Organism Disease Control, Ministry of Agriculture, Qingdao Key Laboratory of Mariculture Epidemiology and Biosecurity, Qingdao 266071, China
Guoliang Yang
College of Life Sciences, Huzhou University, Huzhou 313000, China
Qingli Zhang
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
Covert mortality nodavirus (CMNV), from the Nodaviridae family, is characterized by its unique cross-species transmission and wide epidemic distribution features. In this study, Macrobrachium rosenbergii was proved to be infected naturally by CMNV, which further expand the known host range of CMNV. Here, 61.9% (70/113) of the M. rosenbergii samples collected from Jiangsu Province were CMNV positive in the TaqMan RT-qPCR assay, which indicated the high prevalence of CMNV in M. rosenbergii. Meanwhile, the sequences of CMNV RdRp gene cloned from M. rosenbergii were highly identical to that of the original CMNV isolate from Penaeus vannamei. In situ hybridization (ISH) and histology analysis indicated that the intestine, gill, hepatopancreas and ovary were the targeted organs of CMNV infection in M. rosenbergii, and obvious histopathological damage including vacuolation and karyopyknosis were occurred in the above organs. Notably, the presence of CMNV in gonad alerted its potential risk of vertical transmission in M. rosenbergii. Additionally, numerous CMNV-like particles could be observed in tissues of hepatopancreas and gill under transmission electron microscopy. Collectively, our results call for concern of the potential negative impact of the spread and prevalence of CMNV in M. rosenbergii on its aquaculture, as well as providing a renewed orientation for further investigation and exploration of the diverse pathogenic factors causing M. rosenbergii diseases.
