MicroRNA expression data of pluripotent and somatic cells and identification of cell type-specific MicroRNAs in pigs
Jong-Nam Oh,
Dongchan Son,
Kwang-Hwan Choi,
Jae Yeon Hwang,
Dong-Kyung Lee,
Seung-Hun Kim,
Mingyun Lee,
Jinsol Jeong,
Gyung Cheol Choe,
Chang-Kyu Lee
Affiliations
Jong-Nam Oh
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Dongchan Son
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Kwang-Hwan Choi
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Jae Yeon Hwang
Department of Cellular and Molecular Physiology, Yale School of Medicine, New Haven, CT 06510, USA
Dong-Kyung Lee
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Seung-Hun Kim
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Mingyun Lee
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Jinsol Jeong
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Gyung Cheol Choe
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea
Chang-Kyu Lee
Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea; Designed Animal and Transplantation Research Institute (DATRI), Institute of Green Bio Science and Technology, Seoul National University, Pyeongchang 25354, Korea; Corresponding author at: Department of Agricultural Biotechnology, Animal Biotechnology Major, and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Typical models of pluripotency, humans and mice, have been used to analyse the characteristics of pluripotent stem cells. However, these species exhibit molecular differences in many aspects. With similar physiology and genomics as humans, pigs are promising model for the research of pluripotency. The data of porcine pluripotent cells would be helpful in understanding the molecular network of human pluripotency. Pluripotent cells of humans and mice exhibit specific MicroRNA (miRNA) expression patterns to maintain the pluripotent state. Information about miRNA expression in pig pluripotent cells is not sufficient, so we analysed miRNAs in pluripotent (blastocysts and ES-like) and somatic cell samples (PEB and PFF). We screened cell-type specific miRNAs and identified their target genes. Functional annotation of the target genes was also conducted. Our data may facilitate miRNA-based induction and maintenance of the pluripotent state of porcine cells and provide support to fill the gap between the pluripotency networks of humans and mice.
