Carboxylated Poly-L-lysine Potentially Reduces Human Sperm DNA Fragmentation after Freeze-Thawing, and Its Function Is Enhanced by Low-Dose Resveratrol
Ryota Tachibana,
Hiroki Takeuchi,
Kento Yoshikawa-Terada,
Tadashi Maezawa,
Mikiko Nishioka,
Erina Takayama,
Hiroaki Tanaka,
Kayo Tanaka,
Suong-hyu Hyon,
Yuki Gen,
Eiji Kondo,
Tomoaki Ikeda
Affiliations
Ryota Tachibana
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, Japan
Hiroki Takeuchi
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, Japan
Kento Yoshikawa-Terada
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, Japan
Tadashi Maezawa
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, Japan
Mikiko Nishioka
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, Japan
Erina Takayama
Center of Advanced Reproductive Medicine, Mie University Hospital, 2-174 Edobashi, Tsu 514-8507, Japan
Hiroaki Tanaka
Obstetrics and Gynecology, Mie University Hospital, 2-174 Edobashi, Tsu 514-8507, Japan
Kayo Tanaka
Obstetrics and Gynecology, Mie University Hospital, 2-174 Edobashi, Tsu 514-8507, Japan
Suong-hyu Hyon
BMG, Inc., 45 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto 601-8023, Japan
Yuki Gen
BMG, Inc., 45 Minamimatsunoki-cho, Higashikujo, Minami-ku, Kyoto 601-8023, Japan
Eiji Kondo
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, Japan
Tomoaki Ikeda
Department of Obstetrics and Gynecology, Graduate School of Medicine, Mie University, 2-174 Edo-bashi, Tsu 514-8507, Japan
Sperm DNA fragmentation (SDF) that occurs during the freezing–thawing of sperm may negatively impact the treatment outcomes of assisted reproductive technologies (ART). In a previous study, we developed a human sperm cryopreservation reagent containing carboxylated poly-L-lysine (CPLL) that reduced SDF after freeze-thawing compared with clinically popular cryopreservation reagents containing human serum albumin. However, it is unclear whether CPLL reduces SDF, as it differed from the constituents of the commercial cryopreservation reagents used for comparison. Therefore, here, we examined whether CPLL reduces the SDF of human sperm and evaluated reactive oxygen species (ROS) levels and lipid peroxidation (LPO), which are the causes of SDF; mitochondrial injury, ROS production; and impaired sperm motility. Furthermore, optimal antioxidants and their concentrations that could further enhance the reduction in SDF were determined for future clinical application in ART and underwent the same functional evaluations. CPLL can reduce SDF via inhibition of intracytoplasmic ROS and LPO. Furthermore, the addition of 0.1 mM resveratrol avoided the enhancement of SDF, which potentially affects mitochondrial and cytoplasmic ROS and LPO. This novel human sperm cryopreservation reagent containing CPLL and resveratrol has the potential to improve treatment outcomes in ART using frozen sperm.
