Nature Communications (Jan 2019)

High-throughput single-cell rheology in complex samples by dynamic real-time deformability cytometry

  • Bob Fregin,
  • Fabian Czerwinski,
  • Doreen Biedenweg,
  • Salvatore Girardo,
  • Stefan Gross,
  • Konstanze Aurich,
  • Oliver Otto

DOI
https://doi.org/10.1038/s41467-019-08370-3
Journal volume & issue
Vol. 10, no. 1
pp. 1 – 11

Abstract

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Real-time deformability cytometry (RT-DC) is used for mechanical cell phenotyping but is limited to a single snapshot per cell and can only measure elastic modulus. Here the authors introduce dynamic RT-DC which can measure elasticity and viscosity in single cells by following dynamic cell shape changes.