EVALUATION OF THE EFFECT OF AUXINS ON GROWTH AND BIOCHEMICAL PARAMETERS OF CHAETOCEROS MUELLERI

Abstract

Background. The study of microalgae cultivation process is not only related to obtaining a wide range of biologically active substances. With the development of mariculture, some species of microalgae (including Chaetoceros muelleri) are of interest as food for mollusks, arthropods and holothuria. The aim of this study was to evaluate the effect of auxin hormones on quantitative and qualitative parameters Chaetoceros muelleri accumulative cultivation. Materials and methods. The object of research was the culture of microalgae Chaetoceros muelleri. The duration of the experiment was 7 days. Indole-3- butyric and indole-3-acetic acids were used for growth stimulation. The microalgae were cultured in monoculture, under constant conditions. 0.1; 0.2; 0.4; 0.6; 1.0 ×10-5mol phytohormones concentrations were added to the cultivation medium of the experimental groups. The control group was cultured without phytohormones addition. Results. It was shown that indole-3-acetic acid in concentrations from 0.2 to 0.5×10-5 mol had a positive effect on the growth of algal culture. Auxins at optimum concentration had a positive effect on lipid accumulation in the algal culture. It was found that indole-3-butyric acid increased the carbohydrate content in the culture on the 3rd day of culturing Chaetoceros muelleri. At the same time, under the action of indole-3-butyric acid, the highest content of carbohydrates. Conclusion. The obtained data showed that the studied auxins had a positive effect on Chaetoceros muelleri dynamics growth. The possibility of using auxin series phytohormones to stimulate the growth of the microalga Chaetoceros muelleri in an accumulative monoculture has been established by this study.

Keywords

• chaetoceros muelleri
• auxins
• microalgae
• indole-3-acetic acid
• indole-3-butyric acid