Journal of Mazandaran University of Medical Sciences (Jul 2020)
The effect of mesenchymal stem cell ‑conditioned medium on the proliferation of cancer cell lines, A549 and JEG3
Abstract
Background: Cancer is a significant public health problem. Some studies indicated the anti-cancer effects of mesenchymal stem cells. These effects are related to stem cells or secretory mediator of them. The aim of this study was to evaluate the impact of condition medium of mesenchymal stem cells on A549 and JEG3 cancer cell lines. Methods: In an experimental study, A549 and JEG3 cell lines provided and grown in the culture media of DMEM F12 containing 10% fetal bovine serum were then treated with different percentages of condition medium of mesenchymal stem cells (5, 10, 20, 40, 50, and 100) for 48 hours. Metabolic activity was evaluated by MTT assay in compare to untreated cells (control). Results: MTT assay showed that various percentages of condition medium of mesenchymal stem cell had inhibitory effects on A549 cell line. Significant differences were observed between treated cells to control (p<0.05(.While condition medium had no effects on JEG3 cell line. Conclusion: The results of the present study have shown different effects of condition medium of mesenchymal stem cells on the various cancer cells. According to the inhibitory effects demonstrated in this study on the A549 cell line, proposed that the mesenchymal stem cells and secretory paracrine factors derived them considered as therapeutic targets in these cancers. It should investigate in more studies for confirmation.
