Association between XRCC-1 Arg194Trp polymorphism with lung cancer in Iraqi patients

Abstract

Background: Preface, DNA repair is the most efficient protective approach against DNA damage. Because the integrity of the genome is vital and crucial, the process of repairing DNA induced by carcinogens is critical. The ability to repair DNA damage may be impacted by specific genetic variants in DNA-repair genes, which may also be a risk factor for the development of cancer. The X-ray repair cross complementing-1 (XRCC-1) gene product has been linked to base-excision and single-strand repair mechanisms. Objective: The goal of this study was to examine the relation of the XRCC-1 gene with lung cancer patients. Materials and Methods: polymarease chain reaction- restriction fragment length polymorphism (PCR-RFLP) analysis was used to compare 40 lung cancer patients along with 40 sample controls. Samples were collected at Al-Amal National Hospital for cancer management, ages ranging from 40 to 70 years. Statistics were carried out by using the SPSS Software program Version 20.0. Results: The results show that the XRCC-1 (Arg194Trp) gene carried seven exons, which were shown to have different frequencies in patients and controls based on three genotypes (Arg/Trp, Arg/Arg, and Trp/Trp). Hardy–Weinberg equilibrium with chi-square for the patient (7.949) and control group (8.236). Also, the Arg/Trp genotype was present at a significantly high frequency in patients (77.5%), and the odds ratio was (14.94, confidence interval [CI] = 4.99–44.76 to CI = 0.642), whereas the Trp/Trp genotype frequency increased in the control group (72.5%). And the odds ratio for this relationship was (0.3, P = 0.001, and CI = 0.01–0.11). Conclusion: This study’s findings also suggest that the Arg/Trp genotype may be linked to lung cancer in this particular group.

Keywords

• dna repair
• genotype
• lung cancer
• pcr-rflp
• polymorphism
• xrcc-1 arg194trp