Cell Reports (Jan 2025)
Multi-omics reveals immune response and metabolic profiles during high-altitude mountaineering
- Jianhua Yin,
- Jingzhi Lv,
- Shichen Yang,
- Yang Wang,
- Zhuoli Huang,
- Xue Wang,
- Guixue Hou,
- Wenwen Zhou,
- Ying Liu,
- Weikai Wang,
- Xiumei Lin,
- Yunting Huang,
- Yuhui Zheng,
- Chen Wei,
- Yue Yuan,
- Yaling Huang,
- Chang Liu,
- Haoran Tao,
- Huanhuan Liu,
- Ruquan Liu,
- Yan Zhang,
- Guodan Zeng,
- Feiyun Quan,
- Xinyue Zhu,
- Peng Gao,
- Jun Xie,
- Longqi Liu,
- Jun Cao,
- Chuanyu Liu,
- Xin Jin,
- Jian Wang
Affiliations
- Jianhua Yin
- BGI Research, Shenzhen 518083, China; Shanxi Medical University - BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan 030001, China
- Jingzhi Lv
- BGI Research, Shenzhen 518083, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
- Shichen Yang
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Hangzhou 310030, China
- Yang Wang
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Hangzhou 310030, China
- Zhuoli Huang
- BGI Research, Shenzhen 518083, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
- Xue Wang
- BGI Research, Hangzhou 310030, China
- Guixue Hou
- BGI, Shenzhen 518083, China
- Wenwen Zhou
- BGI Research, Shenzhen 518083, China
- Ying Liu
- BGI Research, Shenzhen 518083, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Hangzhou 310030, China
- Weikai Wang
- BGI Research, Shenzhen 518083, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
- Xiumei Lin
- College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Hangzhou 310030, China
- Yunting Huang
- China National GeneBank, BGI Research, Shenzhen 518120, China
- Yuhui Zheng
- BGI Research, Shenzhen 518083, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
- Chen Wei
- BGI Research, Shenzhen 518083, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China
- Yue Yuan
- BGI Research, Shenzhen 518083, China; BGI Research, Hangzhou 310030, China
- Yaling Huang
- BGI Research, Shenzhen 518083, China; BGI Research, Hangzhou 310030, China
- Chang Liu
- BGI Research, Shenzhen 518083, China; BGI Research, Hangzhou 310030, China
- Haoran Tao
- BGI, Shenzhen 518083, China
- Huanhuan Liu
- BGI Research, Shenzhen 518083, China; BGI Research, Chongqing 401329, China
- Ruquan Liu
- BGI Research, Shenzhen 518083, China
- Yan Zhang
- BGI Research, Shenzhen 518083, China
- Guodan Zeng
- BGI Research, Shenzhen 518083, China
- Feiyun Quan
- BGI Research, Shenzhen 518083, China
- Xinyue Zhu
- BGI Research, Shenzhen 518083, China
- Peng Gao
- Shanxi Medical University - BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan 030001, China; BGI, Shenzhen 518083, China
- Jun Xie
- Shanxi Medical University - BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan 030001, China; MOE Key Laboratory of Coal Environmental Pathogenicity and Prevention, Shanxi Medical University, Taiyuan 030001, China
- Longqi Liu
- BGI Research, Shenzhen 518083, China; Shanxi Medical University - BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan 030001, China; College of Life Sciences, University of Chinese Academy of Sciences, Beijing 100049, China; BGI Research, Hangzhou 310030, China
- Jun Cao
- BGI, Shenzhen 518083, China; Corresponding author
- Chuanyu Liu
- BGI Research, Shenzhen 518083, China; Shanxi Medical University - BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan 030001, China; BGI Research, Hangzhou 310030, China; Corresponding author
- Xin Jin
- BGI Research, Shenzhen 518083, China; Shanxi Medical University - BGI Collaborative Center for Future Medicine, Shanxi Medical University, Taiyuan 030001, China; School of Medicine, South China University of Technology, Guangzhou 510006, China; Corresponding author
- Jian Wang
- BGI Research, Shenzhen 518083, China; James D. Watson Institute of Genome Sciences, Hangzhou 310058, China; Corresponding author
- Journal volume & issue
-
Vol. 44,
no. 1
p. 115134
Abstract
Summary: The physiological perturbations induced by high-altitude exposure in mountain climbers, manifesting as immunological and metabolic deviations, have been previously reported but are not fully understood. In this study, we obtain multi-omic profiles of climbers’ blood samples, including single-cell transcriptomic analysis of 375,722 immune cells, and plasma metabolomics and lipidomics. Longitudinal analysis reveals dynamic immune response profiles, during the acclimatization period, characterized by the downregulation of inflammatory responses in myeloid cell subsets and by the enhancement of immune effector processes in cytotoxic CD8+ T, γδT, and CD16+ natural killer cells. In contrast, during extreme-altitude mountaineering, the activation of inflammatory responses and impairment of immune effector function are observed, concomitant with an increased cellular response to hypoxia and oxidative stress pathways. Furthermore, glycolysis and antioxidant gene expression are upregulated during extreme-altitude mountaineering. Plasma metabolic analysis reveals significant alterations, involving enhanced glutamine and fatty acid metabolism.
