Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis

Dennis Ma; Grace A. Hernandez; Austin E. Y. T. Lefebvre; Hamad Alshetaiwi; Kerrigan Blake; Kushal R. Dave; Maha Rauf; Justice W. Williams; Ryan T. Davis; Katrina T. Evans; Aaron Longworth; Madona Y. G. Masoud; Regis Lee; Robert A. Edwards; Michelle A. Digman; Kai Kessenbrock; Devon A. Lawson

doi:10.1038/s42003-021-02596-y

Communications Biology (Nov 2021)

Patient-derived xenograft culture-transplant system for investigation of human breast cancer metastasis

Dennis Ma,
Grace A. Hernandez,
Austin E. Y. T. Lefebvre,
Hamad Alshetaiwi,
Kerrigan Blake,
Kushal R. Dave,
Maha Rauf,
Justice W. Williams,
Ryan T. Davis,
Katrina T. Evans,
Aaron Longworth,
Madona Y. G. Masoud,
Regis Lee,
Robert A. Edwards,
Michelle A. Digman,
Kai Kessenbrock,
Devon A. Lawson

Affiliations

Dennis Ma: Department of Biological Chemistry, University of California
Grace A. Hernandez: Department of Physiology and Biophysics, University of California
Austin E. Y. T. Lefebvre: Department of Biomedical Engineering, University of California
Hamad Alshetaiwi: Department of Biological Chemistry, University of California
Kerrigan Blake: Center for Complex Biological Systems, University of California
Kushal R. Dave: Department of Physiology and Biophysics, University of California
Maha Rauf: Department of Biological Chemistry, University of California
Justice W. Williams: Department of Biological Chemistry, University of California
Ryan T. Davis: Department of Physiology and Biophysics, University of California
Katrina T. Evans: Department of Physiology and Biophysics, University of California
Aaron Longworth: Department of Physiology and Biophysics, University of California
Madona Y. G. Masoud: Department of Physiology and Biophysics, University of California
Regis Lee: Department of Physiology and Biophysics, University of California
Robert A. Edwards: Department of Pathology & Laboratory Medicine, University of California
Michelle A. Digman: Department of Biomedical Engineering, University of California
Kai Kessenbrock: Department of Biological Chemistry, University of California
Devon A. Lawson: Department of Physiology and Biophysics, University of California

DOI: https://doi.org/10.1038/s42003-021-02596-y
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 15

Abstract

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Ma, Hernandez et al develop a 3D culture-transplant system to enable studies of cancer metastasis using patient-derived xenograft (PDX) tumor cells. Using this system, they find that OXPHOS inhibition attenuates the lung metastatic capacity of breast cancer cells and that overexpression of the metabolic enzyme NME1 increases lung metastasis.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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