Jurnal Farmasi Sains dan Komunitas (May 2014)

ANALISIS KUANTITATIF ISOFLAVON TEMPE SECARA CEPAT DAN SEDERHANA MENGGUNAKAN METODE KROMATOGRAFI LAPIS TIPIS- DENSITOMETRI

  • Agustina Setiawati,
  • Sri Hartati Yuliani,
  • Enade Perdana Istyastono,
  • Michael Rahardja Gani,
  • Evy Fenny Veronica,
  • Dina Christin Ayuning Putri,
  • Reza Eka Putra,
  • David Chandra Putra,
  • Agnes Mutiara Kurniawan

DOI
https://doi.org/10.24071/jpsc.11180
Journal volume & issue
Vol. 11, no. 1

Abstract

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Abstract: Rapid and simple quantitative analysis isoflavones tempe using densitometric TLC has been done. The mobile phase of the system was chloroform: methanol: ethylacetate (45: 5: 0.75). Thin layer chromatography was performed on aluminium TLC plates.Ascending distance of 1 μL sample was performanced 10 cm. Then the plate was scanned at 261 nm. A linear relationship obtained at 0.08 - 2 μg/spot with r= 0.9986. The LOD and LOQ of isoflavone were 0.014 μg/spot and 0.048 μg/spot. Genistein contained in tempe was 0.151± 0.005 % b/b. Keywords: tempe, isoflavon, TLC