Nature Communications (Jun 2021)
Live-cell imaging of circadian clock protein dynamics in CRISPR-generated knock-in cells
- Christian H. Gabriel,
- Marta del Olmo,
- Amin Zehtabian,
- Marten Jäger,
- Silke Reischl,
- Hannah van Dijk,
- Carolin Ulbricht,
- Asylkhan Rakhymzhan,
- Thomas Korte,
- Barbara Koller,
- Astrid Grudziecki,
- Bert Maier,
- Andreas Herrmann,
- Raluca Niesner,
- Tomasz Zemojtel,
- Helge Ewers,
- Adrián E. Granada,
- Hanspeter Herzel,
- Achim Kramer
Affiliations
- Christian H. Gabriel
- Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Laboratory of Chronobiology
- Marta del Olmo
- Institute for Theoretical Biology, Charité Universitätsmedizin Berlin
- Amin Zehtabian
- Institute for Chemistry and Biochemistry, Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin
- Marten Jäger
- Berlin Institute of Health (BIH) Core Genomics Facility, Charité Universitätsmedizin Berlin
- Silke Reischl
- Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Laboratory of Chronobiology
- Hannah van Dijk
- Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Laboratory of Chronobiology
- Carolin Ulbricht
- Immune Dynamics, Rheumatology and Clinical Immunology, Charité – Universitätsmedizin Berlin
- Asylkhan Rakhymzhan
- Biophysical Analytics, Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
- Thomas Korte
- Molecular Biophysics, Department of Biology, Humboldt Universität zu Berlin
- Barbara Koller
- Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Laboratory of Chronobiology
- Astrid Grudziecki
- Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Laboratory of Chronobiology
- Bert Maier
- Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Laboratory of Chronobiology
- Andreas Herrmann
- Molecular Biophysics, Department of Biology, Humboldt Universität zu Berlin
- Raluca Niesner
- Biophysical Analytics, Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute
- Tomasz Zemojtel
- Berlin Institute of Health (BIH) Core Genomics Facility, Charité Universitätsmedizin Berlin
- Helge Ewers
- Institute for Chemistry and Biochemistry, Department of Biology, Chemistry and Pharmacy, Freie Universität Berlin
- Adrián E. Granada
- Charité Comprehensive Cancer Center, Charité Universitätsmedizin Berlin
- Hanspeter Herzel
- Institute for Theoretical Biology, Charité Universitätsmedizin Berlin
- Achim Kramer
- Charité Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Laboratory of Chronobiology
- DOI
- https://doi.org/10.1038/s41467-021-24086-9
- Journal volume & issue
-
Vol. 12,
no. 1
pp. 1 – 15
Abstract
Live-cell recordings have been an important tool for studying circadian rhythms. Here the authors use CRISPR gene editing mediated knock-in to fluorescently tag Per2 and Cry1, and study cellular circadian dynamics of these two clock proteins.
