Disruption of TLE epileptiform activity retarded the seizure and reduced pathological HFOs

Fengru Guo; Airui Li; Qinjun Liu; Daqing Guo; Ke Chen; Dezhong Yao; Yan Cui; Yang Xia

Brain Research Bulletin (Feb 2024)

Disruption of TLE epileptiform activity retarded the seizure and reduced pathological HFOs

Fengru Guo,
Airui Li,
Qinjun Liu,
Daqing Guo,
Ke Chen,
Dezhong Yao,
Yan Cui,
Yang Xia

Affiliations

Fengru Guo: Department of Neurosurgery, Sichuan Provincial People’s Hospital, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China
Airui Li: Department of Neurosurgery, Sichuan Provincial People’s Hospital, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China
Qinjun Liu: Department of Neurosurgery, Sichuan Provincial People’s Hospital, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China
Daqing Guo: Department of Neurosurgery, Sichuan Provincial People’s Hospital, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China
Ke Chen: Department of Neurosurgery, Sichuan Provincial People’s Hospital, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China
Dezhong Yao: Department of Neurosurgery, Sichuan Provincial People’s Hospital, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China
Yan Cui: Corresponding authors.; Department of Neurosurgery, Sichuan Provincial People’s Hospital, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China
Yang Xia: Corresponding authors.; Department of Neurosurgery, Sichuan Provincial People’s Hospital, MOE Key Lab for Neuroinformation, University of Electronic Science and Technology of China, Chengdu 611731, China

Journal volume & issue: Vol. 207
p. 110869

Abstract

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In temporal lobe epilepsy (TLE), the epileptogenic zones, such as the temporal lobe structure, could generate pathological high-frequency oscillations (pHFOs, 250–500 Hz) before the ictal period. These pHFOs have also been observed during the process of seizures in both TLE patients and animals, exhibiting a critical role as promising biomarkers for TLE seizures. TLE seizures could be modulated via regulating the neural excitability in epileptogenic zones, for that TLE is primarily associated with the excitation-inhibition imbalance. However, whether these kinds of modulations could also impact the pHFOs characteristics during TLE seizures is still unclear. For this purpose, we pharmaco-genetically inhibited the principal cells (PCs) in the mouse CA3 region and tracked the difference in the behavioral and electrophysiological features during LiCl-pilocarpine-induced TLE seizure between the hM4Di+CNO (experimental) mice and mCherry+CNO (control) mice. Delayed latency, decreased averaged duration, and reduced counts of the generalized seizure were observed in the experimental mice. Besides, the electrophysiological characteristics, such as the firing rate of PCs and the count of pHFO, exhibited significant decline in the CA3 and CA1 regions. During TLE seizure, there existed strong phase-coupling between pHFO and PCs spike timing in the control mice, while it was abolished in the experimental mice. In addition, we also found that the counts of pHFO were significantly associated with the behavioral features, indicating the close relationships within them. Collectively, our findings suggested that alterations in pHFO and the retardation of seizures may be attributed to disruptions in neuronal excitability, and the variations of electrophysiological features were related to seizure severity during TLE seizures. These results provide valuable insights into the role of pHFOs in TLE and shed light on the underlying mechanisms involved.

Published in Brain Research Bulletin

ISSN: 1873-2747 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Internal medicine: Neurosciences. Biological psychiatry. Neuropsychiatry
Website: https://www.sciencedirect.com/journal/brain-research-bulletin

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