Frontiers in Immunology (Aug 2023)

Interferome signature dynamics during the anti-dengue immune response: a systems biology characterization

  • Júlia Nakanishi Usuda,
  • Desirée Rodrigues Plaça,
  • Dennyson Leandro M. Fonseca,
  • Alexandre H. C. Marques,
  • Igor Salerno Filgueiras,
  • Victor Gabriel Bastos Chaves,
  • Anny Silva Adri,
  • Amanda Torrentes-Carvalho,
  • Mario Hiroyuki Hirata,
  • Paula Paccielli Freire,
  • Paula Paccielli Freire,
  • Rusan Catar,
  • Gustavo Cabral-Miranda,
  • Lena F. Schimke,
  • Lena F. Schimke,
  • Lena F. Schimke,
  • Guido Moll,
  • Otavio Cabral-Marques,
  • Otavio Cabral-Marques,
  • Otavio Cabral-Marques,
  • Otavio Cabral-Marques,
  • Otavio Cabral-Marques,
  • Otavio Cabral-Marques

DOI
https://doi.org/10.3389/fimmu.2023.1243516
Journal volume & issue
Vol. 14

Abstract

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Dengue virus (DENV) infection manifests as a febrile illness with three distinct phases: early acute, late acute, and convalescent. Dengue can result in clinical manifestations with different degrees of severity, dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. Interferons (IFNs) are antiviral cytokines central to the anti-DENV immune response. Notably, the distinct global signature of type I, II, and III interferon-regulated genes (the interferome) remains uncharacterized in dengue patients to date. Therefore, we performed an in-depth cross-study for the integrative analysis of transcriptome data related to DENV infection. Our systems biology analysis shows that the anti-dengue immune response is characterized by the modulation of numerous interferon-regulated genes (IRGs) enriching, for instance, cytokine-mediated signaling (e.g., type I and II IFNs) and chemotaxis, which is then followed by a transcriptional wave of genes associated with cell cycle, also regulated by the IFN cascade. The adjunct analysis of disease stratification potential, followed by a transcriptional meta-analysis of the interferome, indicated genes such as IFI27, ISG15, and CYBRD1 as potential suitable biomarkers of disease severity. Thus, this study characterizes the landscape of the interferome signature in DENV infection, indicating that interferome dynamics are a crucial and central part of the anti-dengue immune response.

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