Frontiers in Pharmacology (Feb 2022)
Relationship Between the Structure and Immune Activity of Components From the Active Polysaccharides APS-II of Astragali Radix by Enzymolysis of Endo α-1,4-Glucanase
- Ke Li,
- Ke Li,
- Ke Li,
- Ke Li,
- Xue-qin Li,
- Xue-qin Li,
- Xue-qin Li,
- Guang-xin Li,
- Lian-jie Cui,
- Lian-jie Cui,
- Lian-jie Cui,
- Xue-mei Qin,
- Xue-mei Qin,
- Xue-mei Qin,
- Zhen-yu Li,
- Zhen-yu Li,
- Zhen-yu Li,
- Yu-guang Du,
- Yue-tao Liu,
- Yue-tao Liu,
- Yue-tao Liu,
- Ai-ping Li,
- Ai-ping Li,
- Ai-ping Li,
- Xing-yun Zhao,
- Xing-yun Zhao,
- Xing-yun Zhao,
- Xin-hui Fan,
- Xin-hui Fan,
- Xin-hui Fan
Affiliations
- Ke Li
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Ke Li
- Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China
- Ke Li
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Ke Li
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- Xue-qin Li
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Xue-qin Li
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Xue-qin Li
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- Guang-xin Li
- College of Agriculture, Shanxi Agricultural University, Taiyuan, China
- Lian-jie Cui
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Lian-jie Cui
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Lian-jie Cui
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- Xue-mei Qin
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Xue-mei Qin
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Xue-mei Qin
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- Zhen-yu Li
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Zhen-yu Li
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Zhen-yu Li
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- Yu-guang Du
- Institute of Process Engineering, Chinese Academy of Sciences, Beijing, China
- Yue-tao Liu
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Yue-tao Liu
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Yue-tao Liu
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- Ai-ping Li
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Ai-ping Li
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Ai-ping Li
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- Xing-yun Zhao
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Xing-yun Zhao
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Xing-yun Zhao
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- Xin-hui Fan
- Modern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, China
- Xin-hui Fan
- The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan, China
- Xin-hui Fan
- Shanxi Key Laboratory of Active Constituents Research and Utilization of TCM, Shanxi University, Taiyuan, China
- DOI
- https://doi.org/10.3389/fphar.2022.839635
- Journal volume & issue
-
Vol. 13
Abstract
Astragali Radix polysaccharides (APSs) have a wide range of biological activities. Our preliminary experiment showed that APS-Ⅱ (10 kDa) was the main immunologically active component of APSs. However, the characteristic structure related to activity of APS-Ⅱ needs further verification and clarification. In this study, APS-II was degraded by endo α-1,4-glucosidase. The degraded products with different degrees of polymerization [1–3 (P1), 3–6 (P2), 7–14 (P3), and 10–18 (P4)] were obtained using a polyacrylamide gel chromatography column. The structural features of the different products were characterized by HPGPC, monosaccharide composition, Fourier transform infrared spectrum, GC–MS, nuclear magnetic resonance, and UPLC-ESI-QTOF-MS analysis. Specific immune and non-specific immune cell tests were used to identify the most immunogenic fractions of the products. The backbone of P4 was speculated to be α-D-1,4-linked glucans and rich in C2 (25.34%) and C6 (34.54%) branches. Immune screening experiments indicated that the activity of P4 was better than that of APS-II and the other three components. In this research, the relationship between the structure of APS-Ⅱ and the immune activity from the degradation level of polysaccharides was studied, laying a foundation for the quality control and product development of APSs.
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