Generation of iPSC line MU011.A-hiPS from homozygous α-thalassemia fetal skin fibroblasts

Amornrat Tangprasittipap; Chonthicha Satirapod; Bunyada Jittorntrum; Sassawat Lertritanan; Usanarat Anurathaphan; Phetcharat Phanthong; Suparerk Borwornpinyo; Narisorn Kitiyanant; Suradej Hongeng

doi:10.1016/j.scr.2015.09.003

Stem Cell Research (Nov 2015)

Generation of iPSC line MU011.A-hiPS from homozygous α-thalassemia fetal skin fibroblasts

Amornrat Tangprasittipap,
Chonthicha Satirapod,
Bunyada Jittorntrum,
Sassawat Lertritanan,
Usanarat Anurathaphan,
Phetcharat Phanthong,
Suparerk Borwornpinyo,
Narisorn Kitiyanant,
Suradej Hongeng

Affiliations

Amornrat Tangprasittipap: Office of Research, Academic Affairs and Innovations, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Chonthicha Satirapod: Department of Obstetrics and Gynecology, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Bunyada Jittorntrum: Office of Research, Academic Affairs and Innovations, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Sassawat Lertritanan: Office of Research, Academic Affairs and Innovations, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Usanarat Anurathaphan: Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand
Phetcharat Phanthong: Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, Thailand
Suparerk Borwornpinyo: Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand
Narisorn Kitiyanant: Stem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, Thailand
Suradej Hongeng: Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand

DOI: https://doi.org/10.1016/j.scr.2015.09.003
Journal volume & issue: Vol. 15, no. 3
pp. 506 – 509

Abstract

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Human iPSC line MU011.A-hiPS was generated from homozygous α-thalassemia (−SEA/−SEA) fetal skin fibroblasts using a non-integrative reprogramming method. Reprogramming factors OCT3/4, SOX2, KLF4, L-MYC, LIN28, and shRNA of TP53 contained in three episomal vectors were delivered using electroporation.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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