Ultra-Sensitive Detection of Chloramphenicol by CdS@NiMoS Nanorods-Based Photoelectrochemical Aptasensor
Hebin Sun,
Yimeng Sun,
Tong Qi,
Zhenyu Wang,
Jianlong Zhao,
Lijuan Liang
Affiliations
Hebin Sun
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
Yimeng Sun
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
Tong Qi
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
Zhenyu Wang
School of Materials Science and Engineering, Harbin Institute of Technology, Harbin 150001, China
Jianlong Zhao
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
Lijuan Liang
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
A novel nanomaterial photoelectrochemical aptamer sensor based on CdS@NiMoS heterojunction nanocomposites was constructed for highly sensitive detection of chloramphenicol (CAP) in antibiotic residues. Through optimization of the material synthesis process, the optimal doping ratio of MoS2 to Ni3+ (70% MoS2 and 10% Ni3+) was identified, which significantly enhanced the photogenerated carrier separation efficiency. In thin-film preparation, comparative analysis of four film-forming methods led to the determination of an optimal process with stability. To achieve highly specific CAP detection, the nanocomposite chip was integrated with nucleic acid aptamer biorecognition elements within a standard three-electrode detection system. Experimental results demonstrated a linear response (R2 = 0.998) in the 0.1–2 μM concentration range, with a detection limit of 3.69 nM (3σ/S).
