Malaysian Journal of Microbiology (Jan 2012)

Isolation and characterization of a new Methanosarcina mazei strain GFJ07 from a mountain forest pond

  • Yi, Y.,
  • Wu, S.,
  • Huang, C.,
  • Cheng, Q.,
  • Rong, Y.,
  • Lan, S.,
  • Li, Y.

Journal volume & issue
Vol. 8, no. 1
pp. 6 – 10

Abstract

Read online

Aims: A new methanogenic strain, named GFJ07, was isolated from a pond of mountain forests in Guangxi, China. Cells grown in liquid culture tended to form aggregates with pseudosarcina-like or irregular shape.Methodology and Results: The optimum temperature, pH and NaCl concentration were 35 ℃, 7.0 and 0.5%, respectively. The isolate used methanol, trimethylamine, acetate and H2-CO2 as substrates. Analysis of the 16S rDNA sequences revealed strain GFJ07 showed the highest sequence similarity of 99.9% to Methanosarcina mazei.Conclusion, significance and impact of study: The cells were Gram positive and nonmotile. Most of single cell grew as a sausage-like clinder about 0.5 μm in diameter and 1.0 μm in length.

Keywords