Measuring expression heterogeneity of single-cell cytoskeletal protein complexes

Julea Vlassakis; Louise L. Hansen; Ryo Higuchi-Sanabria; Yun Zhou; C. Kimberly Tsui; Andrew Dillin; Haiyan Huang; Amy E. Herr

doi:10.1038/s41467-021-25212-3

Nature Communications (Aug 2021)

Measuring expression heterogeneity of single-cell cytoskeletal protein complexes

Julea Vlassakis,
Louise L. Hansen,
Ryo Higuchi-Sanabria,
Yun Zhou,
C. Kimberly Tsui,
Andrew Dillin,
Haiyan Huang,
Amy E. Herr

Affiliations

Julea Vlassakis: Department of Bioengineering, University of California Berkeley
Louise L. Hansen: Department of Bioengineering, University of California Berkeley
Ryo Higuchi-Sanabria: Department of Molecular and Cell Biology, University of California Berkeley
Yun Zhou: Division of Biostatistics, University of California Berkeley
C. Kimberly Tsui: Department of Molecular and Cell Biology, University of California Berkeley
Andrew Dillin: Department of Molecular and Cell Biology, University of California Berkeley
Haiyan Huang: Department of Statistics, University of California Berkeley
Amy E. Herr: Department of Bioengineering, University of California Berkeley

DOI: https://doi.org/10.1038/s41467-021-25212-3
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 14

Abstract

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Existing methods for multimeric protein complex quantification in single cells suffer from limited selectivity and sensitivity. Here the authors report Single-cell protein Interaction Fractionation Through Electrophoresis and immunoassay Readout (SIFTER) and use this to probe the effects of cellular stress.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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