Quantitative volumetric Raman imaging of three dimensional cell cultures

Charalambos Kallepitis; Mads S. Bergholt; Manuel M. Mazo; Vincent Leonardo; Stacey C. Skaalure; Stephanie A. Maynard; Molly M. Stevens

doi:10.1038/ncomms14843

Nature Communications (Mar 2017)

Quantitative volumetric Raman imaging of three dimensional cell cultures

Charalambos Kallepitis,
Mads S. Bergholt,
Manuel M. Mazo,
Vincent Leonardo,
Stacey C. Skaalure,
Stephanie A. Maynard,
Molly M. Stevens

Affiliations

Charalambos Kallepitis: Department of Materials, Imperial College London
Mads S. Bergholt: Department of Materials, Imperial College London
Manuel M. Mazo: Department of Materials, Imperial College London
Vincent Leonardo: Department of Materials, Imperial College London
Stacey C. Skaalure: Department of Materials, Imperial College London
Stephanie A. Maynard: Department of Materials, Imperial College London
Molly M. Stevens: Department of Materials, Imperial College London

DOI: https://doi.org/10.1038/ncomms14843
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

Read online

Advances in chemical imaging capability can add to our understanding of complex cellular systems. Here the authors develop a framework for label-free quantitative volumetric Raman spectroscopic imaging and use it to visualize and quantify biomolecules in various 3D cellular systems.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal