Assessment of the biological activity of Marrubium friwaldskyanum Boiss. (Lamiaceae)
Donika Gyuzeleva,
Tsvetelina Batsalova,
Balik Dzhambazov,
Ivanka Teneva,
Tsvetelina Mladenova,
Rumen Mladenov,
Plamen Stoyanov,
Krasimir Todorov,
Dzhemal Moten,
Desislava Apostolova,
Anelia Bivolarska
Affiliations
Donika Gyuzeleva
Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, 4000, Plovdiv, Bulgaria
Tsvetelina Batsalova
Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Plovdiv, 4000, Bulgaria
Balik Dzhambazov
Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Plovdiv, 4000, Bulgaria; Corresponding author.
Ivanka Teneva
Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, 4000, Plovdiv, Bulgaria
Tsvetelina Mladenova
Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, 4000, Plovdiv, Bulgaria
Rumen Mladenov
Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, 4000, Plovdiv, Bulgaria; Department of Bioorganic Chemistry, Faculty of Pharmacy, Medical University of Plovdiv, 4002, Plovdiv, Bulgaria
Plamen Stoyanov
Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, 4000, Plovdiv, Bulgaria; Department of Bioorganic Chemistry, Faculty of Pharmacy, Medical University of Plovdiv, 4002, Plovdiv, Bulgaria
Krasimir Todorov
Department of Botany and Biological Education, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, 4000, Plovdiv, Bulgaria
Dzhemal Moten
Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Plovdiv, 4000, Bulgaria
Desislava Apostolova
Department of Developmental Biology, Faculty of Biology, University of Plovdiv “Paisii Hilendarski”, Plovdiv, 4000, Bulgaria
Anelia Bivolarska
Department of Medical Biochemistry, Faculty of Pharmacy, Medical University of Plovdiv, 4002, Plovdiv, Bulgaria
Present scientific evidences about the biological activity and potential medical application of extracts derived from Marrubium friwaldskyanum Boiss. are limited. Therefore, our study was undertaken to define several main characteristics in this regard – in vitro cytotoxicity and antitumor properties, antibacterial activity and immunomodulatory potential. Extracts were obtained from different aerial parts of Marrubium friwaldskyanum – stems, leaves and flowers. The in vitro cytotoxicity and antitumor activity of the samples were evaluated by tetrazolium salt reduction tests and Neutral red uptake assays using four human cell lines (a normal fibroblastic and three adenocarcinoma cell lines/A549, HeLa, HT-29/) and by experiments with HT-29 tumor spheroids. Antibacterial activity toward Gram-negative (Escherichia coli) and Gram-positive (Bacillus cereus) species was assessed based on estimation of minimal inhibitory and minimal bactericidal concentrations as well as longitudinal studies on bacterial viability. Ex vivo assays with normal leukocytes were performed to define potential immunomodulatory activity of the extracts. Our results demonstrated selective antitumor activity of the extracts directed against colon adenocarcinoma HT-29 cells and cervical adenocarcinoma HeLa cell line. Metabolic activity of A549 lung adenocarcinoma cells was affected only by the sample derived from flowers. M. friwaldskyanum leaf and flower extracts showed the highest activity, which included reduction of HT-29 tumor spheroid growth and viability. The studied samples exhibited antibacterial activity against both bacterial species tested. Treatment with M. friwaldskyanum extracts affected specific leukocyte populations (HLA+, CD19+, CD11b+, CD25+ cells). These results demonstrate for the first time complex biological effects of extracts derived from M. friwaldskyanum and their potential to serve as a source of valuable compounds for the pharmaceutical industry.
