Head dimensions of brahman and their crossbred bull spermatozoa are affected by cryopreservation

Abstract

The objective of this study was to determine the effect of cryopreservation on morphometrics characteristic of Brahman and their crossbred bull sperm heads. Five ejaculates were collected from 4 bulls and diluted at 30°C in a skim milk-egg yolk extender. Two microscope slides were prepared from single extended sperm samples prior to freezing in nitrogen vapors, and another one after thawing, sperm smears were prepared as described above. All slides were air dried and stained with Hemacolor®. Sperm-head dimensions for a minimum of 150 sperm heads/samples were analysed from each sample by means of the Sperm-Class Analyser ® (SCA), and the mean measurements recorded. A GLM procedure was performed to evaluate the effect of cryopreservation on sperm head morphometric dimensions. Bull sperm heads were significantly (P

Keywords

• cryopreservation
• morphometry
• sperm heads
• Brahman bulls