International Journal of Nephrology and Renovascular Disease (Aug 2020)

Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis

  • Hejazian SM,
  • Ardalan M,
  • Shoja MM,
  • Samadi N,
  • Zununi Vahed S

Journal volume & issue
Vol. Volume 13
pp. 193 – 201

Abstract

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Seyyedeh Mina Hejazian,1– 3 Mohammadreza Ardalan,2 Mohammadali M Shoja,4 Nasser Samadi,1 Sepideh Zununi Vahed2 1Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; 2Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; 3Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; 4Department of Surgery, University of Illinois at Chicago-Metropolitan Group Hospitals (UIC-MGH), Chicago, IL, USACorrespondence: Sepideh Zununi VahedKidney Research Center, Tabriz University of Medical Sciences, Tabriz, IranEmail [email protected]: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients.Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs.Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥ 0.72, P< 0.05).Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.Keywords: nephrotic syndrome, proteinuria, membranous glomerulonephritis, focal segmental glomerulosclerosis, microRNAs, biomarker

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