Development of a sequencing system for spatial decoding of DNA barcode molecules at single-molecule resolution

Abstract

Oguchi et al. developed an in-house sequencing system to spatially decode DNA barcode molecules at single-molecule resolution. They obtain an average read length of 20 nucleotides with an error rate of less than 5% per nucleotide. Leveraging this system, they devised a molecular foot printing method that can be applied spatial proteomics as well as spatial transcriptomics.