PhytoFrontiers (Dec 2024)
A High-Quality Genome Assembly for Aureobasidium pullulans CG163, the Active Ingredient in Aureo Gold, a Bio-Bactericide Registered for Control of Bacterial Canker of Kiwifruit Caused by Pseudomonas syringae pv. actinidiae Biovar 3
Abstract
Aureobasidium pullulans (de Bary) Arnaud (isolate CG163) is a recently commercialized biocontrol agent for the control of Pseudomonas syringae pv. actinidiae (Psa) on kiwifruit (Actinidia spp.). A long-read nanopore assembly, polished with Illumina short reads, resulted in a telomere-to-telomere assembly of CG163. The completed genome has 12 chromosomes ranging from 0.9 to 4.6 Mb and a circular mitogenome of 37.5 kb. A second A. pullulans isolate, which did not inhibit Psa on Actinidia chinensis ‘Hayward’ potted plants (isolate HRY276), was also assembled from long reads for this study. Comparative genomic studies were performed between CG163 and HRY276 (both isolated in New Zealand) and the complete sequence of Aureobasidium pullulans isolate NBB 7.2.1 from Switzerland. There was extensive synteny between the respective chromosomes of all three isolates. The exception was chromosome 12 from CG163, which was not present in the other isolates and may be a dispensable mini chromosome. Mining of the genome of CG163 identified a variety of potential antimicrobial hydrolytic enzymes and secondary metabolite biosynthesis genes that may contribute to the ability of this isolate to suppress Psa on kiwifruit tissues. A complete highly accurate chromosome-scale assembly of CG163’s reference genome will be a valuable resource for future studies investigating the basic underlying molecular genetic mechanisms involved in the biocontrol of Psa, or other plant pathogens, by different isolates of A. pullulans. [Figure: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
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