Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns

Abstract

Biochemical variability between thirteen European and five Czech potato (Solanum tuberosum L.) cultivars grown in theCzechRepublic was studied by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns. It was confirmed that cultivar differences in protein polymorphism can be revealed by applied electrophoretic patterns. It was shown that the different character of protein and isozyme profiles required different approaches to their evaluation. For complex patterns such as electrophoretic soluble protein spectra, it is more convenient to use the evaluation of their absorbance profiles and for simpler profiles of isozymes the evaluation based on the presence or absence of a band in a definite position (simple matching) should be used. In spite of the complexity of tetraploid disposition of analysed cultivars, the results suggested higher similarity of profiles between relative cultivars and they also indicated the existence of higher similarity between cultivars from the same breeding firm.

Keywords

• potatoes
• cultivars
• electrophoresis
• proteins
• isoesterases
• isoperoxidases