Biosensor for Pesticides Based on Valerolacton Copolymer

Abstract

A construction of amperometric biosensor based on immobilized acetycholinesterase and cholin oxidase is described and its application in the detection of organophosphate pesticides through enzyme inhibition measurements is discussed. The bioactive component of the sensor consists of acetycholinesterase or cholin oxidase covalently immobilized on two types new polymeric synthetic membranes. Two types of the copolymers were used for the synthesis of membranes - the copolymer of polyacrylamide and acrylonitrile and the new copolymer of poly- (hexanlactam)-co-block-poly-(delta-valerolactone) with aliphatic polyester. It is investigated the technical characteristics of biosensor like, response time, linear range and operating stability. The factors affecting the inhibition and reactivation processes were investigated too.

Keywords

• Biosensor
• Pecticides
• Enzyme
• Acetylcholinesterase
• Cholin Oxidase
• Paraoxon