Enhancing the 1-Aminocyclopropane-1-Carboxylate Metabolic Rate of <i>Pseudomonas</i> sp. UW4 Intensifies Chemotactic Rhizocompetence

Abstract

1-aminocyclopropane-1-carboxylic acid (ACC) is a strong metabolism-dependent chemoattractant for the plant beneficial rhizobacterium Pseudomonas sp. UW4. It is unknown whether enhancing the metabolic rate of ACC can intensify the chemotaxis activity towards ACC and rhizocompetence. In this study, we selected four promoters to transcribe the UW4 ACC deaminase (AcdS) gene in the UW4 ΔAcdS mutant. PA is the UW4 AcdS gene promoter, PB20, PB10 and PB1 are synthetic promoters. The order of the AcdS gene expression level and AcdS activity of the four strains harboring the promoters were PB20 > PA > PB10 > PB1. Interestingly, the AcdS activity of the four strains and their parent strain UW4 was significantly positively correlated with their chemotactic activity towards ACC, rhizosphere colonization, roots elongation and dry weight promotion. The results released that enhancing the AcdS activity of PGPRenable them to achieve strong chemotactic responses to ACC, rhizocompetence and plant growth promotion.

Keywords

• 1-aminocyclopropane-1-carboxylic acid deaminase
• chemoattractant
• chemotaxis
• metabolic rate
• plant growth promotion
• <i>pseudomonas</i> sp.
• rhizocompetence
• rhizosphere colonization