Angiogenesis by BMP-2-PDLLA-Biohybrids in Co-Culture with Osteoblasts and Endothelia

Abstract

Adsorbate biohybrids for BMP-2 delivery based on electrospun PDLLA-nanofiber fleeces loaded with 0.6 μg/cm2 rhBMP-2COL (E. coli) strongly induced microvessel- like structures when incubated for 14 days in cocultures of human outgrowth endothelial cells (OEC) together with human primary osteoblasts (pOB). Higher loaded rhBMP-2COL biohybrids (4.6 μg/cm2) were low- to noninductive. Adsorbate biohybrids of rhBMP-2CHO (CHO cells) loaded with 1.0-6.0 μg/cm2 induced only a classic cobblestone- like growth pattern without any microvessels. Concentration- response experiments (7 days) indicate a binding affinity of rhBMP-2COL for microvessel induction in the picomolar range with nanomolar concentrations being non-inductive

Keywords

• bmp-2 (e.coli)
• bmp-2 (cho-cells)
• angiogenic sprouts
• proangiogenic effects
• osteogenesis