Optimization of laccase production from Aspergillus flavus by design of experiment technique: Partial purification and characterization

Abstract

The present study describes isolation of laccase producing fungal strain and optimization of the process parameters by design of experiment (DOE) technique to achieve the maximum production of extracellular laccases by Aspergillus flavus obtained from natural habitat. Bromophenol blue dye and ABTS (2,2′-azinobis 3-ethyl-benzothiazoline-6-sulfonate) were used as substrates for the screening of laccase activity. Design expert 8.0.7.1 software was used to optimize culture conditions such as carbon source, nitrogen source, temperature and pH. Subsequently, optimization for inoculums size was also carried out. The optimization studies revealed that the laccase yield was highest when operated at the following conditions: carbon source – cellulose (8%), nitrogen source – peptone (2%), temperature – 35 °C, pH – 7 and inoculum of size 1.5 cm.

Keywords

• Laccase
• Aspergillus flavus
• Bromophenol blue
• ABTS
• Design of experiment