Isolation of Chavibetol and Methyleugenol from Essential Oil of <i>Pimenta pseudocaryophyllus</i> by High Performance Liquid Chromatography
Edenilson dos Santos Niculau,
Leandro do Prado Ribeiro,
Thiago Felipe Ansante,
João Batista Fernandes,
Moacir Rossi Forim,
Paulo Cezar Vieira,
José Djair Vendramim,
Maria Fátima das Graças Fernandes da Silva
Affiliations
Edenilson dos Santos Niculau
Departamento de Química, Universidade Federal de São Carlos (DQ/UFSCar)-Rod. Washington Luís, Km 235, São Carlos CEP 13565-905, SP, Brazil
Leandro do Prado Ribeiro
Centro de Pesquisa para Agricultura Familiar, Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina (CEPAF/EPAGRI)—Rua Ferdinando Ricieri Tusset S/N, São Cristóvão, Chapecó CEP 89801-970, SC, Brazil
Thiago Felipe Ansante
Departamento de Entomologia e Acarologia, Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz” (USP/ESALQ)–Av. Pádua Dias, 11—Agronomia, Piracicaba CEP 13418-900, SP, Brazil
João Batista Fernandes
Departamento de Química, Universidade Federal de São Carlos (DQ/UFSCar)-Rod. Washington Luís, Km 235, São Carlos CEP 13565-905, SP, Brazil
Moacir Rossi Forim
Departamento de Química, Universidade Federal de São Carlos (DQ/UFSCar)-Rod. Washington Luís, Km 235, São Carlos CEP 13565-905, SP, Brazil
Paulo Cezar Vieira
Departamento de Química, Universidade Federal de São Carlos (DQ/UFSCar)-Rod. Washington Luís, Km 235, São Carlos CEP 13565-905, SP, Brazil
José Djair Vendramim
Departamento de Entomologia e Acarologia, Universidade de São Paulo, Escola Superior de Agricultura “Luiz de Queiroz” (USP/ESALQ)–Av. Pádua Dias, 11—Agronomia, Piracicaba CEP 13418-900, SP, Brazil
Maria Fátima das Graças Fernandes da Silva
Departamento de Química, Universidade Federal de São Carlos (DQ/UFSCar)-Rod. Washington Luís, Km 235, São Carlos CEP 13565-905, SP, Brazil
A high performance liquid chromatography (HPLC) method was developed for the simultaneous isolation, on a semi-preparative scale, of chavibetol and methyleugenol from the crude essential oil of P. pseudocaryophyllus leaves. The purity of the isolated compounds and their quantifications were developed using GC/FID. Chavibetol was isolated with high purity (98.7%) and mass recovery (94.6%). The mass recovery (86.4%) and purity (85.3%) of methyleugenol were lower than those of chavibetol. Both compounds were identified on the basis of spectral analysis. The results suggest that the method can provide chavibetol with high purity, mass recovery, and productivity from crude essential, which will be used in bioassays against stored insect pests.
