Agronomía Colombiana (Aug 2010)
Susceptibility of Solanum phureja (Juz. et Buk.) to potato yellow vein virus
Abstract
Sixty two virus-free accessions of Solanum phureja from the Colombian<br />central collection of this tuber (Bogotá-Soacha) were<br />tested for their susceptibility to infection with Potato yellow<br />vein virus (PYVV). Under greenhouse conditions, the plants<br />were inoculated with PYVV using viruliferous Trialeurodes<br />vaporariorum (Westwood) whiteflies, and monitored for three<br />months (the tuber formation period of S. phureja) for symptoms<br />of infection and virus capsid protein (CP) production, by means<br />of a reverse transcription-polymerase chain reaction (RT-PCR)<br />assay. Symptoms of PYVV infection, consisting in the chlorotic<br />flecking of leaflets, followed by complete vein yellowing, were<br />noted in 38 accessions whose RNA extracts generated correctsized<br />CP amplicons in RT-PCR assays. Thirteen of the remaining<br />accessions were found to be latently infected with the virus<br />through positive RT-PCR assays. Twelve accessions were free<br />of virus infection as judged by the absence of symptoms and<br />negative RT-PCR assay results over two cycles of tuber setting<br />and germination. These accessions may possess field resistance<br />to PYVV. This work is the first one to detect and define the levels<br />of susceptibility of Colombian S. phureja accessions to PYVV<br />infection, and to identify the potential existence of resistance<br />to this deleterious Andean potato virus
