Establishment of an induced pluripotent stem cell line DHMi005-A from a healthy male proband

M. Dreßen; T. Luzius; H. Lahm; I. Neb; S.A. Doppler; S. Schneider; E. Dzilic; R. Lange; M. Krane

Stem Cell Research (Mar 2022)

Establishment of an induced pluripotent stem cell line DHMi005-A from a healthy male proband

M. Dreßen,
T. Luzius,
H. Lahm,
I. Neb,
S.A. Doppler,
S. Schneider,
E. Dzilic,
R. Lange,
M. Krane

Affiliations

M. Dreßen: Technical University of Munich, School of Medicine & Health, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636 Munich, Germany; Corresponding author.
T. Luzius: Technical University of Munich, School of Medicine & Health, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636 Munich, Germany
H. Lahm: Technical University of Munich, School of Medicine & Health, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636 Munich, Germany
I. Neb: Technical University of Munich, School of Medicine & Health, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636 Munich, Germany
S.A. Doppler: Technical University of Munich, School of Medicine & Health, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636 Munich, Germany
S. Schneider: Laboratory for Leukemia Diagnostics, Department of Medicine III, University Hospital, LMU Munich, Germany
E. Dzilic: Technical University of Munich, School of Medicine & Health, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636 Munich, Germany
R. Lange: Technical University of Munich, School of Medicine & Health, Department of Cardiovascular Surgery, Institute Insure, German Heart Center Munich, Lazarettstrasse 36, 80636 Munich, Germany; DZHK (German Center for Cardiovascular Research) – Partner Site Munich Heart Alliance, Munich, Germany
M. Krane: DZHK (German Center for Cardiovascular Research) – Partner Site Munich Heart Alliance, Munich, Germany; Division of Cardiac Surgery, Yale University School of Medicine, New Haven, CT, USA

Journal volume & issue: Vol. 59
p. 102662

Abstract

Read online

We generated an induced pluripotent stem cell (iPSC) line from a healthy male 29-year-old proband. Adipose fibroblasts (AFs) were reprogrammed using Sendai virus. Generated iPSCs showed typical stem cell morphology. From passage 9 on, iPSCs were free of virus. Pluripotency in the iPSCs was verified and spontaneous differentiation showed expression of all three germ layers. Karyotyping indicated no anomalies for the generated iPSCs. Many patient-specific iPSCs are generated from subcutaneous fat fibroblasts obtained during surgical procedure. The described control iPSC line was generated equally and therefore serves as an ideal control for adipose-fibroblast-based patient-specific iPSC lines in disease modeling.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal