A Linkage-specific Sialic Acid Labeling Strategy Reveals Different Site-specific Glycosylation Patterns in SARS-CoV-2 Spike Protein Produced in CHO and HEK Cell Substrates

• Qiong Wang,
• Yan Wang,
• Shuang Yang,
• Shuang Yang,
• Changyi Lin,
• Lateef Aliyu,
• Yiqun Chen,
• Lisa Parsons,
• Yuan Tian,
• Hongpeng Jia,
• Andrew Pekosz,
• Michael J. Betenbaugh,
• John F. Cipollo

Affiliations

Qiong Wang

Laboratory of Bacterial Polysaccharides, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Baltimore, MD, United States

Yan Wang

Mass Spectrometry Facility, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD, United States

Shuang Yang

Laboratory of Bacterial Polysaccharides, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Baltimore, MD, United States

Shuang Yang

Center for Clinical Mass Spectrometry, School of Pharmaceutical Sciences, Soochow University, Jiangsu, China

Changyi Lin

Facility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, MD, United States

Lateef Aliyu

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, United States

Yiqun Chen

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, United States

Lisa Parsons

Laboratory of Bacterial Polysaccharides, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Baltimore, MD, United States

Yuan Tian

Laboratory of Bacterial Polysaccharides, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Baltimore, MD, United States

Hongpeng Jia

Department of Surgery, Johns Hopkins University School of Medicine, Baltimore, MD, United States

Andrew Pekosz

Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States

Michael J. Betenbaugh

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD, United States

John F. Cipollo

Laboratory of Bacterial Polysaccharides, Division of Bacterial, Parasitic and Allergenic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Baltimore, MD, United States