Effect of the 60-day introduction of tartrazine and mexidol on the ultrastructure of the rat’s parathyroid gland chief cells

Abstract

Background. The purpose was to study the effect of long-term administration of tartrazine and mexidol on the ultramicroscopic structure of the chief cells of the parathyroid glands in rats. Materials and methods. For the research, 30 mature white male rats were selected and divided into 5 groups: group 1 – rats received daily intragastrically for 60 days 0.9% saline solution. Animals of the group 2 and 3 were injected with the tartrazine solution at a dose of 750 and 1500 mg/kg of body weight under the same experimental conditions and rats of the groups 4 and 5 – 5% mexidol solution intramuscularly at a dose of 50 mg/kg of body weight in order to correct the experimental conditions of the groups 2 and 3. The preparation and processing of parathyroid gland samples were carried out according to the standard protocol for electron microscopic examination. Results. In rats of the groups 2 and 3, electron microscopic signs of a decrease in the secretory activity of the chief cells of the parathyroid glands were revealed, more pronounced in intensity in the group 3. Microscopically, this manifested itself in the raise in the amount of heterochromatin in the nucleus, expansion of cisterns of the rough endoplasmic reticulum, stretched in some places by electron-dense content, a decrease in the content of secretory granules in the cytoplasm, a violation of the morphology of mitochondrial cristae with elements of their destruction, a decrease of intercellular contacts along type of interdigitation. Morphometric study showed a decrease in the volume of the nucleus of the chief cells, the number and average area of secretory granules and mitochondria, compared with the control group. Conclusion. A 60-day administration of tartrazine is accompanied by a change in the ultrastructure of the chief cells of the parathyroid glands of rats, and the administration of mexidol against this background makes it possible to reduce the severity of changes, both qualitative and quantitative, especially in the group using tartrazine at a dose of 750 mg/kg.

Keywords

• parathyroid glands
• chief cells
• ultrastructure
• tartrazine
• mexidol